BES and JMC: The Armed Forces Health Surveillance Division, Global Emerging Infections Surveillance branch (AFHSD-GEIS)-funded surveillance studies. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. OS: Military Infectious Diseases Research Program (MIDRP 2017) D0542_17_AF. Navy Work Unit The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. All other relevant data are within the paper and its Supporting Information files.įunding: FP: The U.S. The work is made available under the Creative Commons CC0 public domain dedication.ĭata Availability: All 20 genomic sequence files are available from the GenBank database under Bioproject ID PRJNA690676, biosample accession number SAMN18394809 to SAMN18394828.
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This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Received: SeptemAccepted: JanuPublished: January 20, 2023 PLoS ONE 18(1):Įditor: Joel Mossong, Health Directorate, LUXEMBOURG (2023) Description of novel capsule biosynthesis loci of Campylobacter jejuni clinical isolates from South and South-East Asia. jejuni isolates.Ĭitation: Khemnu N, Serichantalergs O, Ruekit S, Lertsethtakarn P, Poly F, Swierczewski BE, et al. jejuni multiplex capsule typing system and will help in identifying previously untypeable capsule locus of C. This new primer complements the current C. jejuni in this new group of CPS multiplex assay. The unique sequences provide an additional information of the biosynthesis loci responsible for some of the common CPS sugars/residues such as heptose, deoxtyheptose and MeOPN among C. The designed primers sets were combined in a new multiplex mix called epsilon. Unique PCR primers were designed to identify these new capsule biosynthesis loci.
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The application of the next generation sequencing and whole genome analysis software were used for the identification of novel capsule biosynthesis of C. jejuni by the current multiplex PCR scheme. However, there still are non-typable CPS C. jejuni genome which has been used to develop multiplex PCR to classify CPS types based on the Penner serotypes. The genes responsible for the biosynthesis of the phase variable CPS is located in the hypervariable region of C. jejuni is unusual for an intestinal pathogen in its ability to coat its surface with a polysaccharide capsule (CPS). Campylobacter jejuni is a major cause of bacterial diarrhea worldwide and associated with numerous sequela, including Guillain-Barré Syndrome, inflammatory bowel disease, reactive arthritis, and irritable bowel syndrome.
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